4d stem Search Results


86
ATCC murine embryonic stem cell esc line
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
Murine Embryonic Stem Cell Esc Line, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
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Gatan Inc quantum er dualeels system
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
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Chem Impex International glycerol chem impex
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
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Gatan Inc stemeels tomography
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
Stemeels Tomography, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Chem Impex International adenosine
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
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99
Gatan Inc rotation electron diffraction cred data
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
Rotation Electron Diffraction Cred Data, supplied by Gatan Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Gatan Inc continuum scintillator cmos camera
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
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90
Amaxa 4d-nucleofector basic protocol for human stem cells
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
4d Nucleofector Basic Protocol For Human Stem Cells, supplied by Amaxa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ruska Instrument Corporation 4d-stem
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
4d Stem, supplied by Ruska Instrument Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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90
NION CO 4d-stem
( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 <t>ESC+irradiated</t> 1×10 6 GM-CSF-expressing STO <t>murine</t> <t>embryonic</t> fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.
4d Stem, supplied by NION CO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 GM-CSF-expressing STO murine embryonic fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.

Journal: PLoS ONE

Article Title: Vaccination with Embryonic Stem Cells Protects against Lung Cancer: Is a Broad-Spectrum Prophylactic Vaccine against Cancer Possible?

doi: 10.1371/journal.pone.0042289

Figure Lengend Snippet: ( A ) Bar graph showing GM-CSF expression in non-transduced and retrovirally transduced STO fibroblasts. Error bars represent mean ± SD. *, p<0.05; relative to non-transduced STO cells; t test. ( B ) Scheme of immunization. Male C57BL/6 mice were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 GM-CSF-expressing STO murine embryonic fibroblasts (STO-GM) s.c. in the right flank. Seven days after boost, mice were challenged with 1×10 5 Lewis lung carcinoma cells (LLC) s.c. in the left flank. ( C ) C57BL/6 mice (10/group) were immunized twice (days 0 and 14) with HBSS (control), or irradiated 1×10 6 ESC+irradiated 1×10 6 STO-GM, or irradiated 1×10 6 STO-GM cells alone s.c. in the right flank prior to s.c. challenge with LLC on day 21. Tumor growth was monitored daily in all animals until sacrifice due to tumors exceeding 5% of body weight. The vaccinated tumor free mice remained so for up to 4 months later with no overt signs of distress or autoimmunity. are representative of three independent experiments. **, p <0.001; relative to control group; log-rank test. ( D ). Tumor growth was measured by calipers every 2nd or 3rd day and tumor volumes were plotted as indicated. The data represent the average tumor volumes of 10 mice/control group and 3 mice/ESC/STO-GM group and are representative of three independent experiments. Error bars represent mean ± SEM.

Article Snippet: As a vaccine, we employed the murine embryonic stem cell (ESC) line, ES-D3 (ATCC CRL-11632), derived from 129/Sv mice (expressing MHC class II I-E).

Techniques: Expressing, Control, Irradiation